Proceedings of the XLVII Italian
Society of Agricultural Genetics - SIGA Annual Congress
Verona,
Italy - 24/27 September, 2003
ISBN 88-900622-4-X
Poster
Abstract - 5.40
GENETIC
DIVERSITY IN PHASEOLUS VULGARIS
AND P. COCCINEUS REVEALED BY
PLASTIDIAL AND NUCLEAR MICROSATELLITE MARKERS
L.
NANNI*, D. SICARD*#, J. ACOSTA**, R. PAPA*
*)
Dipartimento di Biotecnologie Agrarie ed Ambientali, Università
Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy
**)
Instituto Nacional de Investigaciones Forestales y Agropecuarias, Chapingo,
México
#Permanent
address : UMR de génétique végétale,
INRA/UPS/CNRS/INA Gif-sur-Yvette, France
Phaseolus
spp., SSR, cpSSR, breeding system, population structure
We investigated on the level and distribution of genetic diversity in the autogamous species P. vulgaris and the allogamous P. coccineus. Two sample of both species representing different geographical scales and different level of sympatry were studied. The first sample contained 67 wild and domesticated accessions from the domestication centres of the two bean species: 24 accessions of P. coccineus from Meso-america; 19 accessions of P. vulgaris from the Andean gene-pool and 24 accessions of P. vulgaris from the Meso-american gene-pool. The second sample contained 115 accessions collected on single plant basis from 6 wild populations: 3 natural populations of P. vulgaris and 3 natural populations of P. coccineus collected in 5 different sites located in the northern area of the state of Morelos, Mexico. In one collection site the two bean species were sympatric. Two types of SSR markers were used: nuclear SSR markers developed from gene sequences including metabolic enzymes and genes related to plant defense and plastidial SSRs (cpSSR) previously shown to be commonly present on the chloroplast genome in gymnosperm and angiosperm. The eight SSR markers previously developed in P. vulgaris, were polymorphic both in P. vulgaris and in P. coccineus. Out of eight cpSSRs tested in P. vulgaris and P. coccineus, three cpSSRs gave polymorphism within and among the two bean species. Results indicate that both nuclear and cpSSR represent a good tool in order to study the genetic diversity in the two Phaseolus species. The population structure, the observed and expected heterozygosity revealed by these markers were in agreement with the contrasting breeding system of P. vulgaris and P. coccineus.