Proceedings of the XLVII Italian Society of Agricultural Genetics - SIGA Annual Congress

Verona, Italy - 24/27 September, 2003

ISBN 88-900622-4-X

Poster Abstract - 2.61

EVALUATION OF THE GENETIC VARIABILITY IN 28 ITALIAN EMMER WHEAT (TRITICUM DICOCCUM SCHUBLER) POPULATIONS BY EST, ISSR AND SSR, MOLECULAR MARKERS

L. MONDINI, M.A. PAGNOTTA, E. PORCEDDU

Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S.C. de Lellis, 01100 Viterbo, Italy

Triticum dicoccum, ISSR, SSR, EST, Variability

Twenty-eight accessions of T. dicoccum have been evaluated by 6 ESTs (DuPw23, DuPw38, DuPw4, DuPw124, DuPw167, DuPw254), 6 SSRs (WMS372, WMS328, WMS294, WMS92, WMS95, WMS319) and 6 ISSRs primers (811, 841, 847, 848, 856, 857) to determine Nei (Nei, 1972) genetic distance and polymorphism.

The evaluated accessions shown great differences in terms polymorphism. The genetic distance, performed with molecular data, cluster the accessions in four groups: i) most of the PLV accessions (5, 6, 7, 8, 9, 11) plus Molise; ii) Garfagnana, Molisano and Padre Pio and with a less clear-cut Farvento Italia Centrale, Abruzzo, Potenza, Umbria and Leonessa; iii) a very uniform group including Andreoli, Loretucci, Carissimi, Achilli, Santoni, Testa, Graziosi and Cicchetti; and iv) a far a part group with Mosè, Lucanica PLV10 and PLV12. Among the PLV group the PLV 10 and 12 are the ones with higher quality characters, while the others are very similar except the yield and plant height. The two not hulled accessions (Mosè and PadrePio) derived from crosses with durum wheat are put in different groups having a Nei genetic distance of 0.29. Even if, on average, polymorphism at DNA level is 15%, the analysed accessions are significant different for the evaluated characters.

Work sponsored by MiPA project "Risorse genetiche di organismi utili per il miglioramento di specie di interesse agrario e per una agricoltura sostenibile".