Proceedings of the XLVII Italian
Society of Agricultural Genetics - SIGA Annual Congress
Verona,
Italy - 24/27 September, 2003
ISBN 88-900622-4-X
Poster
Abstract - 2.58
GENETIC IDENTITY AND SEED PRODUCTION IN
HETEROGENEOUS COMMON BEAN LANDRACES (PHASEOLUS VULGARIS L.) OF “NEW” COMMERCIAL INTEREST
P. MASI, G. LOGOZZO, M.A. DILUCA, G. RAIMONDI, P.L.
SPAGNOLETTI ZEULI
Phaseolus vulgaris, landrace, certification,
genetic markers
Basilicata region
(Southern Italy) is rich of highly variable common bean (Phaseolus vulgaris L.)
landraces that are the subject of increasing economic interests as typical food
productions. The request of larger scale seed production poses a serious risk
of genetic erosion and criteria to monitor and certify the genetic “identity” in
heterogeneous landraces is needed. Two common bean landraces Marrozzo
(indeterminate growth habit) and Verdolino (determinate growth habit), mixtures
of 39 and 22 accessions, respectively, collected in a large area of Basilicata
were grown in two environmentally distinct locations to observe the effect of
growing environment on their genetic structure. 19 morpho-agronomical traits (9
qualitative and 10 quantitative) and 16 microsatellites loci were scored.
On the basis of
morpho-agronomical traits, “Marrozzo”, widely distributed, did not show significantly different response to
different growing environments while Verdolino, for most traits, did show
significant differences.
The 16 pairs of
SSRs primers detected 75 alleles in Marrozzo and 58 alleles in Verdolino with a
polymorphic degree of 100% in Marrozzo and 81.2% in Verdolino. The
heterozigosity average observed value (Ho) is 0.011 in Marrozzo and
0.005 in Verdolino. 7 “unique” alleles were detected in Marrozzo
and 4 in Verdolino with frequency higher of 40%. Genetic identity of each
common bean landrace can be easily assessed on the basis of the presence and
the frequency of unique alleles. To avoid genetic erosion the genetic structure
of landraces during seed production needs to be monitored.