Proceedings of the XLVII Italian
Society of Agricultural Genetics - SIGA Annual Congress
Verona,
Italy - 24/27 September, 2003
ISBN 88-900622-4-X
Poster
Abstract - 2.47
MOLECULAR,
BIOCHEMICAL AND AGRONOMICAL CHARACTERISATION OF TWENTY POPULATIONS OF
‘PEPERONE DI SENISE’ LANDRACE (CAPSICUM ANNUUM L.)
N. BASER*, G.
SONNANTE **, G. LAGHETTI**, G. SARLI**, S. MOLINARI***, G. FANIZZA*
*) Dipartimento
di Biologia e Chimica Agroforestale ed Ambientale; Sez. di Genetica e
Miglioramento Genetico, Facoltà di Agraria di Bari
**) Istituto di
Genetica Vegetale, C.N.R., Bari
***) Istituto
per la Protezione delle Piante Sez. di Bari, C.N.R., Bari
characterization,
‘Peperone di Senise’, Capsicum annuum L., molecular markers,
agronomical traits
In
recent years, conventional agriculture and mechanisation, with the consequent
aggression to the environment due to human activity, have caused a dangerous
genetic erosion, that is a restriction of the variation of plant species and
varieties. Such variation and the development of new local landraces are
important resources of the agriculture in the world. As the increasing genetic
erosion leads to the uniformity of the genetic background of cultivated crops,
the availability of favourable wild genes is becoming crucial for plant
breeding. Therefore, characterisation and evaluation of plant population
collections stored at gene banks is useful to search for those genes which have
adapted in natural conditions to resist pathogens or produce characters of high
quality standards.
Because
of international regulations for restriction of the use of artificial food
stuffs, the interest in pepper as a condiment and natural colorant has steadily
increased. Peppers are rich in carotenoids, which determine the colour of
fruits and in the alkaloid capsaicin, giving the characteristic pungency. These
two parameters of quality are genetically determined and influenced by
environment. The “Peperone di Senise” pepper is the name of a
landrace which has been cropped for many years and adapted very well in the
Italian region of Basilicata. This landrace has been appointed as IGP by the
European Union. In this paper, the agronomic traits of twenty populations of
this landrace are reported along with the quality characters detected by a
spectroscopic analysis of their content in colour and capsaicin; moreover, the
genetic variability of this material has been tested by using RAPD markers.
Agronomic traits were characterised using conventional morphological parameters
from the whole plants, flowers, fruits and seeds. Qualitative analysis focussed
on colour and pungency of fruits; the total extractable colour was assayed and
expressed as ASTA international units per gram of paprika, while capsaicin as
µg/g of paprika. DNA was extracted from a pool of leaves for each
population and RAPD analysis was performed using 20 arbitrary decamer primers.
The
twenty populations were characterized morphologically in detail in three
locations of Basilicata region using 35 descriptors (from ‘Descriptors
for Capsicum’ – IPGRI 1995). In general the results show a wide
phenotypic homogeneity for the whole material even if some populations revealed
distinguishing traits (e.g. pungency, fruit shape, corolla colour, growth
habit, etc.). Interaction among different temperature and methods of drying
with the capsaicin content (almost absent) and the ASTA value (positively
correlated) were tested. In some cases (e.g. ‘Conte’,
‘Gazzaneo’, ‘Forte’ and ‘Amendolara’
populations) a different trend was recorded. These tests were carried out on
fruits with and without seeds and peduncles. Concerning the yield, the best
populations were ‘Forte’ and ‘MG12904’ with 23.9 and 22
t/ha of marketable fruits, respectively. The higher soluble solids content was
reached by the ‘CVM’ population (8.8 %) while the lowest by the
‘CV1’ (5.8%).
In
conclusion, thanks to the data from three locations and different years (to be
completed in the next two years) these twenty populations will be sorted for
yield, yield stability and homogeneity, and fruit quality. A considerable
degree of variation was observed with RAPD markers. A UPGMA dendrogram based on
Jaccard's similarity index allowed to cluster the populations according to
their genetic affinities. Other molecular markers such as AFLP are being tested
in this germplasm.