Proceedings of the XLVII Italian
Society of Agricultural Genetics - SIGA Annual Congress
Verona,
Italy - 24/27 September, 2003
ISBN 88-900622-4-X
Poster
Abstract - 2.43
CONSTRUCTION
OF AN SSR BASED LINKAGE MAP OF DURUM WHEAT
I.
JURMAN*, M.D. CASTELLUCCIO*, M. WOLF**, M.A. OLIVIERI*, E. DEAMBROGIO***, M.
MORGANTE*
*)
Dipartimento Produzione Vegetale e Tecnologie Agrarie, Università degli
Studi di Udine, via delle Scienze 208, 33100 Udine, Tel. +39.0432.558652,
fax +39.0432.558603
**) TraitGenetics GmbH, Am
Schwabeplan 1b, D-06466 Gatersleben Germany
***) Divisione Ricerca, Società Produttori Sementi SpA, via
Macero, 1, 40050 Argelato, Italy
tetraploid
wheat, genetic linkage map, SSRs
We
have initiated the construction of a genetic linkage map of tetraploid wheat Triticum
turgidum L. subsp. durum (Desf.) Husnot
using segregation data from a population of 249 F5 recombinant inbred
lines (RILs) derived from an intraspecific cross between two commercial
varieties of durum wheat. The main durum wheat end-use, in Italy, is pasta, and
high quality pasta is produced employing durum wheat only.
Both
the parents show high pasta-making quality, but one
has a very high protein content and the other has a very high gluten quality.
Microsatellite
markers offer a number of advantages being single locus, co-dominant, highly
polimorphic markers which can be easily mapped. Thus far a total of 129
microsatellite markers derived from hexaploid bread wheat have been analysed.
Linkage
analysis was performed using the MAPMAKER program with the Kosambi mapping
function. A total of 114 loci were mapped at a LOD > 4 on all 14 chromosomes
of the A and B genomes. In general, there is a good agreement between the durum
map obtained and the Triticum aestivum map (Röder et
al, 1998).
This
map will provide a useful tool for detecting economically important
quantitative traits and for marker-assisted selection.