Proceedings of the XLVI Italian
Society of Agricultural Genetics - SIGA Annual Congress
Giardini
Naxos, Italy - 18/21 September, 2002
ISBN 88-900622-3-1
Oral
Communication Abstract - S5e
NITRIC
OXIDE-MEDIATED TRANSCRIPTIONAL CHANGES IN ARABIDOPSIS THALIANA
MOLESINI B.*, DELLEDONNE M.*, BUONAURIO
R.**, MALACARNE G.*, MICA E.*, POLVERARI A.*
*) Dipartimento Scientifico Tecnologico,
Università degli Studi di Verona, Ca’ Vignal 1, Strada Le Grazie
15, 37134 Verona
**) Dipartimento di Arboricoltura e Protezione
delle Piante, Università degli Studi di Perugia, Borgo XX Giugno 74,
06121 Perugia
transcript profiling, cDNA AFLP, cell
death, hypersensitive reaction
Nitric
oxide (NO) production in plants has been shown to be an essential step in
several processes related to development and stress response, with many
important parallels with its role in animal cells. In particular, NO is the
essential molecule that mediates the hypersensitive response of plants to
pathogens, in synergy with reactive oxygen species (ROS). However, the
molecular events downstream of NO still have largely to be elucidated.
Hypersensitive cell death and related resistance responses are active
phenomena, requiring de novo protein synthesis and transcriptional changes
of a large array of genes are likely to have an essential role in the process.
By applying a new cDNA-AFLP technique, we investigated the expression profile
of Arabidopsis thaliana at different times after leaf infiltration with the
NO donor sodium nitroprusside (SNP), which results in an HR-like cell death.
The transcription profiles of about 3.000 genes have been examined, 120 of
which showed an altered expression pattern in comparison to the control
water-infiltrated plants. The large majority (about 98%) of the genes showed a
transcriptional induction, often very early after the treatment (10’).
Comparison of 75 selected sequences with genes reported in genomic and EST
databases revealed homologies with genes involved in a wide range of functions
(basic metabolism, signal transduction, disease resistance, photosynthesis,
etc.), or with predicted proteins of unknown function. Functional genomic
approaches are now undertaken to characterize the role of a few selected genes,
which might have a regulatory role downstream of NO. Our findings indicate a
wide and fast effect of NO on plant transcriptional activity, and confirm
gel-based differential display techniques as an essential tool in new gene
discovery.