Proceedings of the XLVI Italian
Society of Agricultural Genetics - SIGA Annual Congress
Giardini
Naxos, Italy - 18/21 September, 2002
ISBN 88-900622-3-1
Oral
Communication Abstract - S1c
ABIOTIC AND BIOTIC STRESS FACTORS
AFFECT THE GERANYL GERANYL HYDROGENASE EXPRESSION IN PEACH LEAVES (PRUNUS
PERSICA [L.] BATSCH)
GIANNINO D.*, CONDELLO E.**, BRUNO L.***, TESTONE G.
**, COZZA R.***, TARTARINI A**, NICOLODI C.**, BITONTI M.B.***, INNOCENTI
A.M.***, MARIOTTI D.**
*) Istituto per la Protezione delle Piante –
Sezione di Bari, CNR, Via Amendola trav.147,16815 Bari
**) Istituto di Biologia Agro-ambientale e Forestale.
Sezione di Roma, CNR, Via Salaria km 29,300, 00016 Monterotondo Scalo, Roma,
Tel 06 90672539 Fax 06 9064492
***) Dipartimento di Ecologia
dell’Università della Calabria, Ponte Bucci, 87030 Arcavacata di
Rende, Cosenza, Tel 0984 492965, Fax 0984 492964
peach, geranyl geranyl
hydrogenase, stress response
A previous research
showed that the gene encoding geranyl geranyl reductase (GGH), a key enzyme of
the chlorophyll and tocopherol pathways, was regulated at the developmental
level in peach leaves. In this work, GGH expression was monitored in response
to light absence, cold treatment, wound injury and during the leaf curl disease, caused by
the fungus Taphrina deformans. Mature and young leaves were subjected to
dark and GGH messages appeared to decrease after 12 hrs, but they partially recovered
with respect to controls in long last treatments (36 hrs). A similar pattern
was observed in mechanically injured leaves, which exhibited a rapid decrease
and recovery of GGH messages within only four hours from the treatment.
Moreover, in plants maintained at 4°C, after growth chamber acclimation,
leaf GGH expression was progressively down regulated within 24 hours. In curled
leaves message decrease was tightly related to the damage intensity.
Interestingly, in situ experiments revealed a strong transcript accumulation
in distinct leaf portions at the early symptoms of disease. This pattern
suggested that GGH was locally triggered in response to the pathogen
induced oxidative stress by taking part in the tocopherol pathway.