Proceedings
of the XLVI Italian Society of Agricultural Genetics - SIGA Annual Congress
Giardini Naxos, Italy - 18/21
September, 2002
ISBN 88-900622-3-1
Poster
Abstract - 4.21
THE USE OF
TETRA-PRIMER ARMS-PCR FOR GENOTYPING SINGLE NUCLEOTIDE POLYMORPHISMS (SNPs) IN
BARLEY
CHIAPPARINO E.*, LEE D.*, TUBEROSA R.**
*)
NIAB, Huntingdon Road, Cambridge CB3 0LE, UK
**)
Dipartimento di Scienze e Tecnologie Agroambientali, Università di
Bologna, Italy
barley,
SNP, molecular markers,varieties, genotyping
Single nucleotide polymorphisms (SNPs)
are the most abundant form of DNA polymorphism. Their use as molecular markers
is gaining interest because they have great potential for applications in plant
breeding programmes, for the identification of genetic loci affecting traits of
interest, and for the characterisation and exploitation of genetic resources.
In addition, a dramatic increase in the
number of DNA sequences submitted to databases is facilitating the
identification of SNPs in many species, while the single most important factor
preventing their widespread development and use appears to be the cost.
A simple and economical method involving
a single PCR reaction is reported here for barley SNPs genotyping. Using the
tetra-primers ARMS-PCR procedure (Ye et al., 2001), we have been able to
unambiguously assay SNPs in a set of varieties of cultivated barley. The
results show the reliability, low cost and ease of use of this technique.