Proceedings of the XLVI Italian
Society of Agricultural Genetics - SIGA Annual Congress
Giardini
Naxos, Italy - 18/21 September, 2002
ISBN 88-900622-3-1
Poster
Abstract - 3.10
Comparative phylogeny of Anthyllis spp. between nuclear ITS and chloroplast
SSR
NANNI
L., FERRADINI, E., BELLUCCI E., PAPA R.
Dipartimento
di Biotecnologie Agrarie ed Ambientali, Università degli Studi di
Ancona, Ancona, Italy
Anthyllis-phylogeny-phenetic
and cladistic analysis, infinite allele model, stepwise mutational model
Our
previous results showed that the genus Anthyllis (Fam. Fabaceae,
tribe Loteae, 2n=2x=12) is not monophyletic and it is divided in two main
clades: the vulneraria and tetraphylla clade. The first
includes most of the Anthyllis species and the second, several
species more closely related to Lotus.
To clarify
the relationships among the different species both phenetic and cladistic
analysis were conducted on ITS sequence data and compared with the phylogeny
obtained with chloroplast microsatellites.
Chloroplast SSR data were analysed following both
the infinite allele model and the stepwise mutational model to verify which one
was suitable for analysing the phylogenetic relationships among Anthyllis spp.. Our analyses show
that also cpSSRs are powerful
phylogenetic markers for both inter and intra specific studies in the genus Anthyllis. In
this genus the molecular diversity of cpSSR does not fit the stepwise
mutational model because the main part of the observed variation appears to
result from indels in regions flanking the repeat motif.