Proceedings of the XLVI Italian Society of Agricultural Genetics - SIGA Annual Congress

Giardini Naxos, Italy - 18/21 September, 2002

ISBN 88-900622-3-1

Poster Abstract - 3.03

Automated karyotype analysis, DNA content and rDNA sequences in different accessions of VICIA MICHAUXII Sprengel

Ravalli C.*, Frediani M.**, Ruffini Castiglione M.***, Cremonini R.***, Venora G.*

*) Stazione Sperimentale di Granicoltura per la Sicilia, Caltagirone

**) D.A.B.A.C. Università della Tuscia

***) Dip. Scienze Botaniche Università Pisa

Vicia michauxii Sprengel; CHIAS; Chromosomes; DNA content; rDNA sequences

In a recent classification, Maxted et al. (1991) subdivided the genus Vicia into two subgenera, namely Vicia and Vicilla, with 17 and 9 sections respectively.

Recently Jaska (1997) analysed the isoenzyme diversity and phylogenetic affinities of species of the subgenus Vicia, and he evidenced close relationships among V. faba and the species of the Peregrinae section (Vicia michauxii, Vicia peregrina, Vicia aintabensis according to Maxted, 1994). Furthermore, Jasha (1997), highlighted that the species belonging to the Narbonensis complex which are considered to be closest wild relatives of cultivated bean by many traits similarities, constitute a separate monophyletic group.

More over recent RFLP and RAPDs analysis of nuclear and mitochondrial DNA has also suggested more close affinities of Vicia faba to the section Peregrinae than to the Narbonensis complex (Van de Ven et al. 1993). This result implies that, contrary to previous views (Zohary and Hopf 1973) the faba bean has not been domesticated from any of the Narbonensis complex but rather from an unknown ancestor common with the section Peregrinae.

The recorded results based on isoenzymes, nuclear and mitocondrial DNA show only preliminary hypotheses of phylogenetic affinities and estimates of genetic divergence inside the subgenus Vicia

In order to get more information we have started to study different species of Peregrinae section (Cremonini et al. 2001).

In the present paper we report cytological and molecular results on seven accessions of Vicia michauxii and one subspecies namely V. michauxii sub. (var.) stenophylla. By cytological analysis it is possible to share each accessions and subspecies into different groups on the bases of morphology of satellited pairs. The analysis of DNA sequences has revealed a different organisations.

The results are discussed on the phylogentic point of view.

{Przybylska-Zimniak & Przybylska 1995 9452 /id}