Proceedings of the XLVI Italian
Society of Agricultural Genetics - SIGA Annual Congress
Giardini
Naxos, Italy - 18/21 September, 2002
ISBN 88-900622-3-1
Poster
Abstract - 2.08
A BROAD APPROACH TO STUDY GRAPEVINE GENE
EXPRESSION
MOSER
C., GATTO P., SEGALA C., FONTANA P., PINDO M., VELASCO R.
Area
Biologia Avanzata; Istituto Agrario S. Michele all’Adige, Via Mach 1, S.
Michele all’Adige, Trento
grapevine,
gene expression, ESTs, arrays, resveratrol
Gene
expression analysis on a genome-wide scale has opened new possibilities to
answer complex biological questions. The microarray technology and the
high-throughput sequencing are the main technical bases of such a revolution in the experimental scale. In the
last few years, though later than the human- and the biomedical-, also the
plant biology field has been pervaded by this significant advance.
Grapevine
(Vitis vinifera L.) is today one of the most widely cultivated and economically
important crop due to its large adaptability to different climates and soils.
As for most woody plants also for grape, gene expression studies are very
limited in number and almost exclusively focused on one tissue, namely the
berry. In order to gain novel insights at the molecular level into essential
physiological processes like photosynthesis, plant defense and biosynthesis of
secondary metabolites, we started one year ago a functional genomics project.
Starting point was the construction of cDNA liraries from different grapevine
tissues: leaf, inflorescence, sprout, bud, berry and root. Sequencing of clones
randomly picked from the libraries has yielded up to now 1920 ESTs (Expressed
Tagged Sequences) from inflorescence and
sprout (cv. Regent) and 750 ESTs from leaf (cv. Pinot noir). The
sequence information was then processed via bioinformatics tools in order to
assign a putative function to each clone and to build a grapevine searchable
database. In parallel, spotting of the amplified ESTs on to membrane filter or
glass support will permit true genome-wide sampling of gene expression
patterns. Preliminary experiments to compare the gene expression profiles in
young, fully developed and senescent leaves by cDNA-macroarrays are under way.
Within the functional genomics project an important and complementary role is played by the development of differential cDNA libraries obtained by subtractive hybridization among cDNAs pools coming from different tissues or different cultivars. First goal of these experiments will be to highlight different transcript profiles between grapevine cultivars with high and low resveratrol content. Polyphenols are becoming more and more attractive for their properties in pharmacology and nutrition. Our purpose is then to dissect the metabolic pathways involved into their accumulation in plant tissues.