Proceedings of the XLVI Italian Society of Agricultural Genetics - SIGA Annual Congress

Giardini Naxos, Italy - 18/21 September, 2002

ISBN 88-900622-3-1

Poster Abstract - 1.28

FINGERPRINTING CHERRY CVS BY A RETROTRANSPOSON BASED MOLECULAR MARKERS

VARASANO E.*, PORCEDDU A.*, LANFALONI L.**, BALDONI L.*

*) CNR-Istituto Ricerche Miglioramento Genetico Piante Foraggere, Via Madonna Alta 130, 06126 PG

**) Dipartimento Biologia Cellulare e Molecolare, Via Elce di Sotto, PG

cherry biodiversity, retrotransposon, REMAP, IRAP

In recent year, the generation and display of DNA fingerprints have proven extremely valuable for biodiversity applications. The dispersion, ubuquity and prevalence of retrotransposon in plant genomes provide an excellent basis for development of a marker system. By using a PCR based protocol with degenerated primers a cherry genomic library enriched for LTR-like sequences was constructed. Three out of five sequenced clones showed high homology with reverse transcriptases of Ty copia retrotransposons.

Based on the available sequence information, primers specific for one LTR sequence were designed and used in REMAP (Retrotransposon Microsatellite Amplified Polymorphism) and IRAP ( Inter Retrotransposon Amplified Polymorphism). Only few bands were amplified using the IRAP protocol, suggesting that retrotransposon are not clustered in the cherry genome. REMAP protocol, produced a complex banding pattern which was readily distingioshible from taht of ISSR.

The level of polymorphism detectede by REMAP marker was, in general, higher than ISSR suggesting that this marker system is highly proficient for biodibersity studies.