Proceedings of the XLVI Italian Society of Agricultural Genetics - SIGA Annual Congress

Giardini Naxos, Italy - 18/21 September, 2002

ISBN 88-900622-3-1

Poster Abstract - 1.02

CHARACTERIZATION OF GENE REPERTOIRES DURING DEVELOPMENT OF CITRUS FRUITS

BORTESI L., MAROCCO A.

Istituto di Botanica e Genetica Vegetale, Università Cattolica del Sacro Cuore, Piacenza

We carried out a random sequencing of cDNAs derived from immature and mature citrus fruit (Citrus sinensis) to identify the gene repertoires expressed during development. A technique of RNA fingerprinting was optimized allowing the detection of DNA fragments derived from RNA using cDNA synthesis and subsequent PCR amplification. RNAs were isolated from three cultivars with different level of pigmentation and at three developmental stages. Thirty different primer combinations, each of the primer carrying a selective base at 3’ end, were employed in PCR assay to amplify the sequences generated by restriction reaction with BstYI and MseI. Sequencing and homology search in DNA database is beginning, from which genes related to fruit development will be identified.