Proceedings of the XLV
Italian Society of Agricultural Genetics - SIGA Annual Congress
Salsomaggiore Terme, Italy -
26/29 September, 2001
ISBN 88-900622-1-5
Poster Abstract
THE HYBRID ORIGIN OF
THE BLACK FLOWERED POPPY
FERRADINI
N., BELLUCCI E., D’AMICO T., SAGRATI G., SANPIETRO E.
Dipartimento di
Biotecnologie Agrarie ed Ambientali, Università degli Studi di Ancona,
Ancona, Italy
A Papaver spp. plant with black
colored flower has been discovered along the littoral of the Senigallia coast
(Italy). From the progeny of this plant the new variety "Evelina" has
been developed and patented.
Considering that the
color of the petals is genetically stable, two hypothesis have been proposed to
account for the presence of this novel character: a mutation or a spontaneous
interspecific hybridization.
In order to discriminate
between these two hypothesis and
identify the parental origin, the variety Evelina, 4 Papaver species present in the
area as wild or cultivated (P. dubium, P. ibridum, P. roheas, P. somniferum), and the hybrid
(Evelina x P. roheas var. shyrley) were compared for morphological traits and
molecular markers. Overall, 61 individuals were studied.
From the morphological
evaluation, the new variety
"Evelina" presented intermediate characters between P. roheas and P. dubium.
The analysis of the
genetic diversity was performed using ISSR markers which combine
characteristics like universality, high number of amplified loci, variability
and reproducibility that made them particularly appropriate to study inter and
intraspecific variability. Fifty-three polymorphic ISSR markers were analysed.
UPGMA cluster analysis revealed that among the all species studied P. rhoeas and P. dubium were the most closely
related to the black poppy.
The PCR amplification of
the ribosomal DNA (ITS, 18S-5.8S and ITS2, 5.8S-25S) followed by a restriction
digestion with the enzymes Eco RV and Bam HI generates polymorphism that
confirmed the hypothesis of the hybrid origin of the black poppy, and P. roheas has been identified as
one of the parental species. Moreover, analysis of the chloroplast genome with
eight universal cpSSR primers has identified P. dubium as the maternal parent.