Proceedings of the XLV Italian Society of Agricultural Genetics - SIGA Annual Congress

Salsomaggiore Terme, Italy - 26/29 September, 2001

ISBN 88-900622-1-5

Poster Abstract

ADVANCED BACKCROSS QTL ANALYSIS IN DH LINES DERIVED FROM AN H. VULGARE X H. SPONTANEUM CROSS

TALAMÈ V.*, CHIAPPARINO E.*, BAHRI H.**, BEN SALEM M.***, TERIACA M.*, THIS D.****, FORSTER B.P.*****

* Dipartimento di Scienze e Tecnologie Agroambientali, Università di Bologna, Italy

t_vale@hotmail.com

** Ecole National D’Agricolture de Meknes, Morocco

*** Institut National de la Recherche Agronomique de Tunisie, Tunisia

**** INRA-Montpellier, France

***** Scottish Crop Research Institute, Dundee, UK

barley, SSR, QTL analysis, drought stress

Wild barley (Hordeum spontaneum L.) is a valuable source of genes to improve biotic and abiotic stress tolerance. An Advanced Backcross QTL Analysis (ABQA) approach has been utilised to overcome problems associated with wild gene introgression, such as linkage drag and background effects associated with QTL detection. Doubled haploid lines (DHs) were obtained by means of anther culture from F2BC1 plants derived from the cross between a donor accession of H. spontaneum and a European recipient cultivar (Barke). The DH lines characterised by negative agronomic traits such as ear shattering, excessive earliness and lateness, low kernel weight and/or dormancy were not considered. Sixty two (45 pair of SSR primers provided by Scottish Crop Research Institute and 24 from Liu et al) pairs of SSR primers were used to analyse the parental lines; 35 SSRs which showed polymorphisms were used to genotype the 130 selected DH lines. In addition, 3 AFLP primer combinations (PCs) have also been utilised; these PCs revealed a total of 295 bands, 35 of which were found polymorphic. A further set of SSRs is currently being tested. Phenotypic evaluation of the DH lines is in progress in two trials conducted in Morocco and Tunisia under drought stress conditions and in a third trial carried out near Bologna (Italy). QTL analysis will be performed to identify molecular markers associated with QTLs for the main morpho-physiological traits and for grain yield.